|
KMID : 0620920110430040189
|
|
Experimental & Molecular Medicine
2011 Volume.43 No. 4 p.189 ~ p.196
|
|
|
Hydroxydibenzoylmethane induces apoptosis through repressing ornithine decarboxylase in human promyelocytic leukemia HL-60 cells
|
|
Wang Ming-Fu
Liao Ya-Fan
Hung Ying-Cheng
Lin Chih-Li
Hour Tzyh-Chyuan
Lue Ko-Huang
Hung Hui-Chih
Liu Guang-Yaw
|
|
|
Abstract
|
|
|
|
Ornithine decarboxylase (ODC) is the rate-limiting enzyme in polyamine biosynthesis and a target for chemoprevention. Hydroxydibenzoylmethane (HDB), a derivative of dibenzoylmethane of licorice, is a promising chemopreventive agent. In this paper, we investigated whether HDB would inhibit the ODC pathway to enhance apoptosis in human promyelocytic leukemia HL-60 cells. We found ODC enzyme activity was reduced during HDB treatment. Overexpression of ODC in HL-60 parental cells could reduce HDB-induced apoptosis, which leads to loss of mitochondrial membrane potential (Δψ m), through lessening intracellular ROS. Furthermore, ODC overexpression protected cytochrome c release and the activation of caspase-3 following HDB treatment. The results demonstrated HDB-induced apoptosis was through a mechanism of down-regulation of ODC and occurred along a ROS-dependent mitochondria-mediated pathway.
|
|
|
KEYWORD
|
|
|
apoptosis, hydroxydibenzoylmethane, mitochondrial membrane potential, ornithine decarboxylase, reactive oxygen species
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|